More information

Tatiana Sandoval Guzmán Group

Vertebrate tissue repair and regeneration

Portrait Tatiana Sandoval Guzmán

The vertebrate limb contains various tissue types including bone, skin, nerves, muscle and blood vessels. After an injury these tissues respond to a plethora of signals driving repair and, in few animal species, regeneration. Our main goal is to understand how individual tissues respond to an injury to further understand their interaction during axolotl limb regeneration. The fine-tuned coordination of both, the individual tissue regeneration and the interaction with other tissue types could be the key for successful appendage regeneration. Moreover, we aim to find differences and similarities between axolotl and mammalian regeneration, in order to identify key components that promote or restrict regeneration in mammals.

Previous and current research

Figuring out how to unlock the inherent regeneration potential in mammals and specifically in humans requires a practical model of regeneration. Axolotl is a powerful model where transgenesis has proven useful for studying molecular and cellular mechanisms of regeneration. Another advantage of this animal model is its semitransparent body, allowing intra-vital imaging of the limb. Axolotl represents a simplified organism in comparison with mammals, yet more complex than other animal models, that is uniquely suited to study bone formation, and appendage phenotypes. 

Tatiana Sandoval Guzmán Research: Figure
Tissue reorganization in an axolotl digit at day 2 and day 13 during regeneration. A transgenic line expressing multiple fluorescent proteins, shows the cellular dynamics of connective tissue, and allows us to follow individual cells in real time.

Future Projects and Goals

The research in our laboratory explores the capacity of different organisms to regenerate missing or damaged components. Various tissue types respond to a plethora of signals initiating proliferation and migration triggered by injury, and in some animal species this will lead to regeneration. Our research interest encompasses three different aspects of regeneration:

  1. Contextual regeneration: although some mechanisms are conserved, a different response could be trigger depending on the microenvironment. This is determined for example, by tissue type, position in the body, and size of the injury.
  2. Physiological adaptation: to understand how an organism accommodates a lengthy regenerative process, we study changes in different metabolic organs and circulating factors.
  3. Cross-species comparison: we aim to identify key components that promote or restrict regeneration in mammals.

To answer these questions, we alternate between a highly regenerative organism and those with very limited regeneration. This facilitates bridging our discoveries and understanding key mechanisms of successful regeneration.

Methodological and Technical Expertise

  • Axolotl histology
  • In vivo imaging of axolotls
  • Murine whole-embryo culture
  • Mammalian digit regeneration
  • Molecular biology of tissue regeneration


since 2022
Research group leader, PLID/Medical Faculty, Technische Universität Dresden, Germany

Research group leader, CRTD, Technische Universität Dresden, Germany

Postdoctoral Fellow, DFG-Center for Regenerative Therapies Dresden, Technische Universität Dresden

Postdoctoral Fellow, Karolinska Institute, Sweden

Ph.D., University of Arizona, USA

Selected Publications

Riquelme-Guzmán C, Schuez M, Böhm A, Knapp D, Edwards-Jorquera S, Ceccarelli AS, Chara O, Rauner M, Sandoval-Guzmán T
Postembryonic development and aging of the appendicular skeleton in Ambystoma mexicanum
Dev Dyn. (2021)

Currie, JD, Grosser L, Murawala P, Schuez M, Michel M, Tanaka EM, Sandoval-Guzmán T
The Prrx1 limb enhancer marks an adult population of injury-responsive, multipotent dermal fibroblasts Biology Open 8(7) (2019)

Sandoval-Guzmán T, Currie JD
The journey of cells through regeneration Current Opinion in Cell Biology, 55:36–41 (2018)

Khattak S, Murawala P, Andreas H, Kappert V, Schuez M, Sandoval-Guzmán T, Crawford K, Tanaka EM
Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination
Nature Protocols, 9(3):529–40 (2014)

Sandoval-Guzmán T, Wang H, Khattak S, Schuez M, Rönsch K, Nacu E, Tazaki A, Joven A, Tanaka EM, Simon A
Fundamental differences in dedifferentiation and stem cell recruitment during skeletal muscle regeneration in two salamander species
Cell Stem Cell 14(2):174–87 (2014)