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Portrait Ellen Adams

Liquid-liquid phase separation (LLPS) occurs in living cells, in which liquid-like membrane-less compartments form. These protein enriched biomolecular condensates regulate biochemical and biophysical processes, but have been linked to formation of protein aggregates found in neurodegenerative diseases. LLPS stems from intrinsically disordered proteins (IDPs), where protein-protein interactions between regions of low complexity have been shown to be a major driving force. However, little is known about the role of the water solvent in this process. Why do two coexisting liquid phases that are entropically unfavorable form? How do properties of water inside biomolecular condensates differ from water outside? How do protein-water interactions, i.e. hydration water, contribute to driving LLPS?

Our group studies macroscopic and microscopic solvent properties of biomolecular condensate systems. We use linear and nonlinear spectroscopic methods to probe the structure and dynamics of bulk and interfacial water. We aim to understand the underlying thermodynamic properties of protein-water interactions that lead to LLPS.

Ellen Adams Research: Figure
Figure: We investigate solvent properties in biomolecular condensate systems. With THz spectroscopy, we get a molecular level picture of protein hydration water confined in biomolecular condensates.

Future Projects and Goals

  1. Hydration properties of biomolecular condensates
    Proteins have extended dynamic hydration shells that are fundamental to their structure and function. Using THz spectroscopy, we probe the structural properties of protein hydration water and determine how this drives biomolecular condensate formation.
  2. Solvent dynamics of protein systems
    Formation and aging of biomolecular condensates are dynamical processes in which material properties are altered. We use pump-probe spectroscopy to determine how solvent dynamics are correlated to macroscopic changes within protein systems.   
  3. Interfacial properties of biomolecular condensates
    Biomolecular condensates are membrane-less organelles, but little is known about the molecules that comprise this interface. With the use of the surface selective sum frequency generation spectroscopy, we aim to unravel a molecular picture of these interfaces.

Methodological and Technical Expertise

  1. THz-ATR spectroscopy
  2. THz time domain spectroscopy
  3. Pump-probe spectroscopy
  4. Sum Frequency Generation Spectroscopy

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Ellen Adams is currently offering PhD positions

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Open Project
  • Prevention of Intrinsically Disordered Protein Aggregation with Solvent Tuning: Unraveling the Role of Water in Condensate Aging
    Preferred Course of Study/Expertise of Candidate: Molecular Biology, Biochemistry, Chemistry, Physics
    → Detailed information as PDF

CV

Since December 2021
Research Group Leader at Cluster of Excellence Physics of Life, TU Dresden and Helmholtz Zentrum Dresden-Rossendorf (HZDR)

2017–2021
Postdoctoral Researcher, Ruhr-Universität Bochum, Germany

2017
Postdoctoral Researcher, The Ohio State University, USA

2016
PhD Physical Chemistry, The Ohio State University, USA

More Information

Ellen Adams at TUD PoL

Selected Publications

E.M. Adams*, H. Hao*, I. Leven, M. Rüttermann, H. Wirtz, M. Havenith, T. Head-Gordon
Proton Traffic Jam: Effect of Nanoconfinement and Acid Concentration on Proton Hopping Mechanism
Angew. Chem. Int. Ed. 60(48), 25584 (2021)

E.M. Adams, S. Pezzotti, J. Ahlers, M. Rüttermann, M. Levin, A. Goldenzweig, Y. Peleg, S.J. Fleishman, I. Sagi, M. Havenith
Local Mutations Can Serve as a Game Changer for Global Protein Solvent Interaction
JACS Au, 1(7), 1076–1085 (2021)

J. Ahlers*, E.M. Adams*, V. Bader*, S. Pezzotti, K.F. Winklhofer, J. Tatzelt, M. Havenith
The Key Role of Solvent in Condensation: Mapping Hydration Water in Liquid-Liquid Phase-Separated FUS
Biophysical Journal, 120(7), 1266 (2021)

E.M. Adams, O. Lampret, B. König, T. Happe, M. Havenith
Solvent Dynamics Player a Decisive Role in the Complex Formation of Biologically Relevant Redox Proteins
Phys. Chem. Chem. Phys., 22, 7451–7459 (2020)

E.M. Adams, C.B. Casper, H.C. Allen
Effect of Cation Enrichment on Dipalmitoylphosphatidylcholine (DPPC) Monolayers at the Air-Water Interface
J. Colloid Interface Sci., 478, 353–364 (2016)

*equally contributed