More information

Simon Alberti Group

Organization of cytoplasm across space and time

Portrait Simon Alberti

© Katrin Boes

Many key biochemical reactions take place in the cytoplasm. We still know very little about the organization of the cytoplasm and the role of biomolecular condensates such as RNP granules in regulating cellular functions. My research group aims to elucidate molecular principles underlying the spatiotemporal organization of the cytoplasm by condensate formation. We are particularly interested in understanding how the cytoplasm changes upon environmental perturbations and stress (Figure 1). Stressed cells undergo controlled changes on many levels to alter their physiology and metabolism. Many of these changes result directly from alterations in the structure and organization of the cytoplasm. Understanding these changes and how they promote organismal survival is our key aim.

To investigate this question, we use cell biological, biochemical, biophysical and genetic approaches and diverse model systems, such as yeast, Dictyostelium, and cultured mammalian cells, allowing us to cover a wide range of organismal life styles. Our findings so far indicate that the mechanisms by which macromolecules form condensates are diverse and involve dedicated factors such as prion-like proteins and scaffold molecules such as RNAs.

Importantly, the ability to form such condensates comes with a cost, as many condensate-forming proteins have a high propensity to misfold and aggregate. Indeed, we could recently show that condensate-forming proteins have very unusual molecular properties and are associated with age-related diseases (Patel et al., 2015; Maharana et al., 2018). Thus, our long-term aim is to gain insight into the important link between the condensate-forming abilities of proteins and age-related protein misfolding diseases such as amyotrophic lateral sclerosis.

Simon Alberti research: figure
Figure 1: Research focus of the Alberti lab. The figure shows an idealized cell, which transitions into a different state upon stress. This transition is associated with changes in the organization of the cytoplasm and the formation of large macromolecular assemblies. In normal cells, the macromolecules are not interacting with each other (unassembled). However, upon stress, these macromolecules interact and form assemblies with specific material properties (liquid or solid condensed matter state). Thus, the overall process has hallmarks of a phase transition. Stress-induced assemblies can either function as storage depots or as compartments for concentrated biochemical reactions.

Future Projects and Goals

  • Identify protein factors, domains, and sequence motifs that are required for the formation of condensates
  • Identify molecular mechanisms regulating the formation of condensates, with a focus on the protein quality control machinery and post-translational modifications
  • Genetic and chemical screens to identify modifiers of condensate formation
  • Investigate how the ability to form condensates affects the physiological state of a cell, determines developmental decisions, and contributes to diseases and aging

Methodological and Technical Expertise

  • Genetics and cell biology of S. cerevisiae, Dictyostelium, and cultured mammalian cells
  • Fluorescence microscopy and time-lapse imaging
  • Biophysical approaches such FRAP and photoconversion to study intracellular dynamics
  • Biochemical reconstitution assays and in vitro and in vivo aggregation assays

Apply now!

Simon Alberti is recruiting in the current PhD application round.

Apply now!

CV

Since 2018
Professor of Cellular Biochemistry at BIOTEC Dresden

2010–2018
Group leader at the Max-Planck-Institute of Molecular Cell Biology and Genetics in Dresden

2005–2009
Post-doctoral Fellow at the Whitehead Institute for Biomedical Research in Cambridge (USA)

2004
PhD in Biology, University of Bonn

More Information

www.biotec.tu-dresden.de

Selected Publications

Simon Alberti, Amy Gladfelter, Tanja Mittag
Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates.
Cell, 176 pp. 419–434 (2019)

Shovamayee Maharana, Jie Wang, Dimitrios Papadopoulos, Doris Richter, Andrei I. Pozniakovsky, Ina Poser, Marc Bickle, Sandra Rizk, Jordina Guillén-Boixet, Titus Franzmann, Marcus Jahnel, Lara Marrone, Young-Tae Chang, Jared Sterneckert, Pavel Tomancak, Anthony Hyman, Simon Alberti
RNA buffers the phase separation behavior of prion-like RNA binding proteins.
Science, 360 pp. 918–921 (2018)

T. M. Franzmann, M. Jahnel, A. Pozniakovsky, J. Mahamid, A. S. Holehouse, E. Nüske, D. Richter, W. Baumeister, S. W. Grill, R. V. Pappu, A. A. Hyman, Simon Alberti
Phase separation of a yeast prion protein promotes cellular fitness.
Science, 359, eaao5654 (2018)

Jie Wang, Jeong-Mo Choi, Alex S Holehouse, Hyun O. Lee, Xiaojie Zhang, Marcus Jahnel, Shovamayee Maharana, Regis P. Lemaitre, Andrei I. Pozniakovsky, David N. Drechsel, Ina Poser, Rohit V Pappu, Simon Alberti, Anthony Hyman
A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins.
Cell, 174 (2018)

Avinash Patel, Hyun-Ok Kate Lee, Louise Jawerth, Shovamayee Maharana, Marcus Jahnel, Marco Y Hein, Stoyno Stoynov, J. Mahamid, Shambaditya Saha, Titus Franzmann, Andrei Pozniakovski, Ina Poser, Nicola Maghelli, Loic Royer, Martin Weigert, Eugene W Myers, Stephan W. Grill, David N. Drechsel, Anthony Hyman, Simon Alberti
A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation.
Cell, 162, pp. 1066–1077 (2015)

Contact

Biotechnology Center of the TU Dresden (BIOTEC)
Tatzberg 47/49
01307 Dresden